Biological and Genetic Characterization of Canine Distemper Virus Vaccine Candidate Named as CD1901-100

Canine distemper virus (CDV) infections cause high morbidity and mortality in dogs. Changes the molecular biological characteristics of Korean CDV strain over multiple cell passages have not been reported. We investigated genetic CD1901-100 for use as an inactivated vaccine strain. Vero cells expressing dog nectin-4 gene (Vero/dNectin-4 cells) were used to adapt CD1901, which was passaged 100 times four types cells. assessed cytopathic effects immunofluorescence assays identify features CD1901 CD1901-100. Seven explore tropisms two strains. The analyses based on whole-genome sequencing data. signaling lymphocyte activation molecule infected with strains showed different fluorescence properties. attained highest viral titer 106.5 TCID50/mL at 4 days post-inoculation; overall 107.0 that after growth Vero/dNectin-4 exhibited 25 nucleotide mutations 15 amino acid substitutions six structural proteins compared sequences. Of proteins, F protein had number replacements (5/663, 0.75%). constructed a line propagated well This will aid development vaccine.